bioTRADING products - Identification & Confirmation Tests - Ready prepared media is our culture!

Why test for Campylobacter?
Campylobacter is recognized by food safety regulators as one of the most important contaminants of food and is the leading bacterial cause of human gastroenteritis. Campylobacter infections can be fatal. Low infective dose: as few as 500. Campylobacter cells can cause sickness.

Poultry and raw milk are the main source of Campylobacter. Thus, dairy products, poultry and ready-to-eat meals have been the main causes of outbreaks in recent times. Water contaminated with Campylobacter has also led to Campylobacteriosis.

Processed poultry products can also be prone to post-processing contamination. Campylobacter will not grow below 30°c. However, they will survive better under chilled conditions than at ambient temperatures, and can multiply once the food is removed from refrigeration. Accordingly, it is very important to ensure that chilled foods are free of Campylobacter.

The new rapid test method makes testing for Campylobacter simple, fast, accurate, reliable and economical.

Unique™ Campylobacter
Fast, Convenient, Reliable Campylobacter Detection.

Fast

One of the fastest tests available for Campylobacter. From sample to result in just over 42 hours compared to 4 days for conventional methods!
Using the UNIQUE PLUS™, only minimal labour time is required. Simply add enrichment sample to the module, place into the UNIQUE PLUS and wait for the results!

Cost Effective

Rapid results ensure product can be released sooner, reducing holding costs.

Reliable

Extensively tested for a wide range of food and environmentalsamples
High sensitivity: 1-5 cfu / 25 g sample
Highly purified antibodies enable the user to detect the presence of all Campylobacter species

Safe

Safer than some standard enrichment methods which require bubbling gas through media to create a low oxygen environment. This has the potential to create harmful aerosols
Uses a heat-killed enrichment sample, thereby reducing the risk of infection
Blood-free enrichment medium

Easy

Proprietary Enrichment medium permits aerobic incubation. Thus, no micro-aerophilic incubation system required
A Single Enrichment Step required for most foods

Convenient

All reagents provided in ready-to-use, self-contained modules
Each module can be used manually for a single test, or automated using the UNIQUE PLUS instrument for up to 30 tests per run
Reduced labour time
Compact module for easy storage
Support tray for handling and storing modules

Campylobacter Visual Immunoassay
A rapid and specific test for the detection of Campylobacter spp. in food and environmental samples.

Fast

One of the fastest tests available for Campylobacter. Results can be obtained within 2 hours following enrichment.
Approximately 42 hours to obtain a positive or negative result compared to a minimum of 4 days to clear a negative sample with conventional methods.

Simple

A single enrichment step.
No microaerophilic incubation required.
All reagents provided.
Easy-to-read results.
Blood-free enrichment medium.
No membrane strip which may become clogged with sample residue and affect the test result.

Accurate & Reliable

Unique high affinity antibodies and specially-formulated Campylobacter Enrichment Broth give results in which you can have confidence. Sensitivity : 1-5 cfu / 25 g of sample

Economical

The simplicity of the method frees up laboratory time for other tasks and projects. More can be accomplished with the same labor.
Rapid results ensure inventory can be released earlier, reducing holding costs.

Proven Performance

The method has been validated in independent trials.
Comparative studies showed performance equivalence to standard cultural methods.

Flexible

Recommended for a wide range of food, water and environmental samples.
Depending on your specific needs, the kit can be used manually or automated for large scale testing.

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Captivate™

Captivate™ E.coli
Captivate™ Salmonella

Captive™ is a range of antibody coated paramagnetic particles for the specific ImmunoMagneticSeparation of microorganisms.

This technology consists of microscopic paramagnetic particles. The beads have a magnetite core and a “ceramic” zirconium oxide coating. The beads are manufactured by high speed blending process and typically cover a size diameter range of 1-4 µm, with a 2.5 µm average size.

Purified antibodies to surface components of the target microorganism are covalently coupled to the bead. With careful antibody selection, a highly specific separation system for microorganisms is produced.

The pre-coated beads are designed for the IMS of target bacteria from enrichment cultures. A sample is taken form a filter stomacher bag and incubated with the Captive™ beads for 30 minutes. The bead/microoragnism complexes are then removed from the sample by placing the sample in a magnetic concentrator device. This separates them form the background organisms and interfering materials. The complexes are then washed using a PBS/Teen 20 wash buffer to remove non-specifically bound material. The beads can then be plated out onto the appropriate selective agar media and incubated as described.

The IMS technique will increase the sensitivity of the methodology and, in most circumstances, results can be achieved 24 hours earlier than standard protocols.

These products can also serve as a capture system for rapid detection systems.

Special Notes on IMS Techniques.
There are important factores that affect the performance of IMS techniques. Thorough mixing of the particles and sample allied with efficient recovery of the beads from the sample matrix is paramount to the success of this technique. Care must be taken not to aspirate the sample vigorously as this can result in the loss of captured target organisms. Certain sample types (e.g. very fatty, particulate and viscous samples) can interfere with bead recovery. To counter-act this interference, samples can also be diluted in PBS-Tween e.g. 1:2-1:4, reducing the effect of the matrix and allowing more efficient bead recovery. Alternatively with problem samples, after the initial magnetic separation the incomplete removal of the sample (i.e. remove 800 µl) and continuation of the wash protocol as described can minimise bead losses.

Captivate™ E.coli

Captive™O157
Captive™O157 are magnetisable particles coated with specific antibody intended for the isolation of E.coli O157:H7 from food, animal feeds, beverages, pharmaceutical or environmental samples. The particles help to concentrate O157:H7 cells in mixed culture reducing the probability of missing low numbers or overgrowth of O157:H7 colonies by competing flora. In fact, immunomagnetic separation is now regarded as the gold standard method for isolation of E.coli O157:H7 from food and environmental samples.

E.coli O157:H7 is the primary serovar associated with food borne gastrointestinal infection, resulting in self-limiting diarrhoea, that can lead to serious disease conditions such as haemorrhagic colitis, haemolytic uraemic syndrome and thrombotic thrombcocytopaenic purpura (TTP).

The organism itself is associated with raw meats and unpasteurised milk, probably due to the implication of farm animals and particularly cattle as carriers of E.coli O157:H7. Large outbreaks have been recorded in the United States from consumption of unpasteurised apple juice (apple cider) possibly as a result of using apples which have fallen to the ground where the potential for contamination with the organism exists.

Also available

Captivate™O26
Captivate™O111
Captivate™O103
Captivate™O145

Captivate™ Salmonella
This product is designed to capture and concentrate the common serotypes of Salmonella involved in human and animal disease from enriched samples. The particles are coated with affinity purified plyclonal antibody directed towards, common somatic and flagellar antigens. This gives an excellent high avidity broad spectrum IMS reagent fro the capture of salmonellas. Due to the large variation in Salmonella serotypes and antigen expression there is naturally strain dependent variation in the capture efficiency.

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Chlamydia

Direct Fluorescent Antibody kit

Direct Fluorescent Antibody kit
A direct fluorescent antibody kit using a monoclonal antibody produced to recognise the major outer membrane of all 15 serotypes of Chlamydia trachomatis. The antibody is conjugated with fluoroscein and stains Chlamydia trachomatis elementary bodies and reticulate bodies. The reagent does not react with Chlamydia psittaci. The kit is supplied in a 60 test format complete with mounting media and a positive/negative control slide.

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E. coli

E. coli O157 Latex kit, E. coli O157:H7 Latex Test Reagent 2 ml
E. coli O157 Visual Immunoassay

Why test for E. coli O157?
E. coli O157 is regarded as one of the most serious threats to the producers and consumers of food, all over the world. Food poisoning outbreaks, caused by E. coli O157, have involved a wide range of foods including meat and meat products, milk and dairy products, vegetables, salads and fruit juices.

Outbreaks involving E. coli O157 have resulted in major product recalls and substantial economic loss. Numerous cases of illness and many deaths have resulted.

The USDA has issued an E. coli O157:H7 test directive to producers, retailers and
importers/exporters of raw ground beef products.

E. coli O157 Latex kit, E. coli O157:H7 Latex Test Reagent 2 ml
The innovative E.coli O157 latex kit offers definitive identification of E.coli O157 through the use of a proprietary antibody that eliminates the possibility of cross-reactions with Escherichia hermanii and other sorbitol-negative Escherichia species.

So sensitive, only a single, direct colony is required
Rapid results in only 2 minutes!
Presented in a uniquely designed, user friendly workstation
Available in two convenient sizes of 50 or 100 tests
Contains positive and negative control
Incredible 2 year shelf life!

Prolex™ E. coli H7 Flagellar Antigen Latex Reagent
The use of the Prolex™ H7 reagent allows immediate identification and confirmation of positive E. coli O157:H7 organisims.

E. coli O157 Visual Immunoassay
A rapid and specific test for the detection of E. coli O157 in food and environmental samples.

Fast

Using the E. coli O157 Visual Immunoassay, you can:
Screen out negatives within 20 hours, compared to 2 days for standard cultural methods
Ship product sooner based on a rapid method result

Reliable

The E. coli O157 VIA can be used to comply with the USDA’s E. coli O157:H7 test directive.
Highly purified antibodies give results in which you can have confidence
Sensitivity : 1-5 cfu / 25 g sample False positives : <1%
Specificity : >99% False negatives : <1%
Studies have shown the method to be more sensitive than comparative rapid methods
Recommended for a wide range of foods and environmental samples

Convenient

All reagents provided
Easy-to-read results
Proven performance for use with composite 375 g ground beef samples
Heat treating samples ensures E. coli O157 are killed and safe to work with, unlike magnetic bead technology
The simplicity of the E. coli O157 VIA method frees up laboratory time for other tasks and projects
Rapid results ensure inventory can be released earlier, reducing holding costs
Depending on your specific needs, the kit can be used manually or automated for large scale testing

Proven Performance

Comparative studies show performance that is equivalent to standard cultural methods

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Enterotoxin

Unique™ Staphylococcal Enterotoxin
Staphylococcal Enterotoxin Visual Immunoassay
Staphylococcal Enterotoxin Identification VIA™
Bacillus Diarrhoeal Enterotoxin Visual Immunoassay

Why test?
Staphylococcal food poisoning is one of the most common types of foodborne disease worldwide. It is also one of the most economically important diseases, estimated to cost the United States approximately $1.5 billion each year.

Some strains of S.aureus are capable of producing a highly heat-stable protein toxin that causes illness in humans. Heat treatments commonly used in food processing may kill viable S.aureus cells but cannot be relied upon to inactivate enterotoxins. Therefore, it is advisable that foods be examined for the presence of residual toxin in addition to viable organisms.

Food poisoning outbreaks caused by S.aureus have been associated with a wide range of foods including meat and meat products, poultry and egg products, milk and dairy products, salads, bakery products, sandwich fillings and fast foods. Some foods are particularly susceptible to growth of S.aureus during production, including pasta, some cheeses, fermented sausages, ham and salami.

Unique™ Staphylococcal Enterotoxin
SETUNQ™ is a novel method for fast, convenient, and reliable detection of Staphylococcal Enterotoxins A, B, C1, C2, C3, D and E in food, food related samples and enrichment cultures.

Fast
Detects pre-formed Enterotoxins in 4 hours
Screen for presence of Enterotoxin-producing Staphylococcus spp. in less than 22 hours
Rapid results ensure that product can be released sooner, reducing holding costs

Reliable

Extensively tested for a wide range of food and environmental samples
Detects the seven key Staphylococcal Enterotoxins – A, B, C1, C2, C3, D and E.
High sensitivity: detection limit demonstrated to be as low as 0.25 ng / ml of sample extract.

Convenient

Simple extraction step
All reagents provided in ready-to-use self-contained test modules
Each module can be used manually for a single test; or automated using the UNIQUE PLUS™ instrument for up to 30 tests/run
Reduced labour time
Compact module for easy storage

Built-in positive and negative controls give you confidence!
Trial Parameters: 65 food samples
Toxins for Spiking: SEB & SEC1
3 spiking levels: Unspiked
Low level – 0.25 ng / ml
High level – 1.0 ng / ml

Outcome: The UNIQUE Staphylococcal Enterotoxin method demonstrated agreement with the US FDA BAM Reference Method @ 1.0 ng / ml and sensitivity down to 0.25 ng / ml

Staphylococcal Enterotoxin Visual Immunoassay
A rapid and simple screening test for indicating the presence of Staphylococcal Enterotoxins (SET) A, B, C1, C2, C3, D and E in food, food-related samples and enrichment cultures.

Fast
Using the Staphylococcal Enterotoxins Visual Immunoassay, you can:

Detect pre-formed SET in food within 4 hours.
Screen food and environmental samples for the presence of enterotoxin-producing Staphylococcus spp. in as little as 22 hours.

Reliable

High affinity antibodies give results in which you can have confidence.
High sensitivity: <1 ng / ml of prepared sample.

Convenient

All reagents provided ready-to-use.
Easy-to-read results.
Simple extraction step.
The simplicity of the Staphylococcal Enterotoxins VIA frees up laboratory time for other tasks and projects.
Depending on your specific needs, the kit can be used manually or automated for large scale testing.

Proven Performance
Recommended for a wide range of food and food-related samples
The Staphylococcal Enterotoxins VIA method has been extensively validated in independent trials.

Enterotoxins Identification
Following positive results from the Staphylococcal Enterotoxins VIA, individual toxins can be identified using the Staphylococcal Enterotoxins Identification VIA.

Staphylococcus aureus Screening
The Staphylococcus aureus VIA can be used alongside the Staphylococcal Enterotoxin VIA to screen for viable S.aureus cells.

Staphylococcal Enterotoxin Identification VIA™
A rapid, convenient and specific identification test for use following a positive result being obtained with the SETVIA™ or SETUNQ™. Toxins A, B, C, D and E can be specifically identified.

Results in 4 hours
72 well ELISA format
All reagents provided

Bacillus Diarrhoeal Enterotoxin Visual Immunoassay
A rapid and simple screening test for indicating the presence of Bacillus Diarrhoeal Enterotoxin (BDE) in food and food-related samples, and enrichment cultures.

Why are BDE-Producing Bacillus spp. considered a significant problem to the food industry?

Bacillus cereus and other Bacillus spp. Are frequently found in a very wide range of foods, including baby foods, cooked and frozen rice products, cereals, meat products, soups, milk and milk powders, and extended shelf-life cooked-chill meals.
Food poisoning outbreaks involving B. cereus have resulted in major product recalls and substantial economic loss. In the USA published data indicates that B. cereus causes up to 84,000 cases of food poisoning each year.
The heat resistant nature of Bacillus spores allows them to survive in many foods, which have undergone moderate heat processing and normal cooking processes.

The Bacillus Diarrhoeal Enterotoxin method can be used in 2 ways:
1. To detect the presence of pre-formed BDE in food within 4 hours.
2. To detect the production of BDE after overnight enrichment of samples containingenterotoxigenic Bacillus spp.

Why is there a need to test for pre-formed Bacillus Diarrhoeal Enterotoxin in food?

Whilst the pre-formed BDE has been shown, in some cases, to be degraded in the stomach of adults, young children do not possess this ability. Thus outbreaks of Bacillus Diarrhoeal food poisoning due to pre-formed toxin have usually involved children.

Why test for Bacillus Diarrhoeal Enterotoxin after overnight enrichment of samples?

Various species of Bacillus occur commonly in food. Thus it is important to have a test that can detect Bacillus isolates that have the ability to cause Bacillus Diarrhoeal food poisoning.
BDE-producing Bacillus spp. may only produce a detectable level of BDE after a prolonged growth period. The level of pre-formed toxin in a food sample may be below a detectable level. However, the food may contain viable Bacillus, and if ingested, these organisms may produce enterotoxin and cause food poisoning.

Fast

Detect pre-formed BDE in only 4 hours!
You can ship product sooner based on a rapid result.
Screen food and environmental samples for the presence of Enterotoxin-producing Bacillus spp. in less than 20 hours.

Accurate & Sensitive

The method has shown excellent correlation with biological assays and epidemiological evidence and no non-specific interference from a wide range of food products has been observed.
Sensitivity : >1 ng / ml of prepared sample

Simple and Convenient

You can save time and money. Minimal handson time compared with traditional methods (e.g. immunogel diffusion) which are extremely labor-intensive, complex to perform and require extensive reagent preparation.
Up to 46 tests can be performed simultaneously.

Proven Performance

Verified to accurately identify BDE in food artificially inoculated with Diarrhoeal. Enterotoxin and in food and faecal samples from actual food poisoning incidents.

Flexible

Recommended for a wide variety of food and food-related samples.
Depending on your specific needs, the kit can be used manually, semi-automated, or fully automated for large scale testing.

Economical

The simplicity of the method frees up laboratory time for other tasks and projects. More can be accomplished with the same amount of labor.
Rapid results ensure inventory is released earlier, reducing holding costs

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Gram Negative Biochemical ID Test System
For the identification of Enterobacteriaceae and Listeria spp.

The Gram Negative Biochemical ID Test System can be used to confirm the identity of Enterobacteriaceae and other non fastidious, oxidase negative, Gram negative bacilli, such as Salmonella spp., isolated from food and environmental sources.

The Listeria Biochemical ID Test System can be used to confirm the identity of Listeria spp.

The ID kits can be used to identify pure cultures but are also useful for the identification and confirmation of isolates that have produced presumptive positive results in any of Salmonella or Listeria rapid screening kits.

Simple
The ID kits employ just 12 standardised microwell substrates. They are simple to use, with no assembly required, single colony inoculation and non-toxic reagents. The Identification System Software can be used to provide a reliable identification.

Accurate
The selection of the 12 substrates is based on extensive computer analysis of published databases developed over numerous decades for the identification of commonly encountered food pathogens and spoilage organisms.

Current Database
One of the major challenges facing microbiologists is keeping up to date with changes in taxonomy. All recent changes are incorporated into the Identification System Software.

Convenient
The kits are conveniently sized and contain all the reagents required. Each test is presented in a compact microwell format and requires fewer manipulations and less hands-on time than other methods.

Economical
ID kits can save time and money, eliminating the need to make up numerous types of biochemical test media.

Flexible
A single software package for both ID kits.

Proven Performance & Reliability
Comparative studies show greater accuracy and reliability than other biochemical ID kits currently on the market

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Legionella

Prolex Legionella Latex Kit
Prolex Legionella Individual Latex Reagents
Legionella pneumophila FITC conjugated antibodies

Prolex Legionella Latex Kit
bioTRADING offers a kit employing a monoclonal antibody, specifically directed against surface antigens on Legionella pneumophila serogroup 1, eliminating cross reactions with other pneumophila and non pneumophila serogroups as well as other species of bacteria. The kit is supplied complete with test latex, positive and negative controls, reaction cards and mixing sticks, sufficient for 20 tests.

Prolex Legionella Individual Latex Reagents
A full range of Legionella Individual Latex Reagents:

L. pneumophila serogroup 2, polyclonal 50 tests
L. pneumophila serogroup 3, polyclonal 50 tests
L. pneumophila serogroup 4, polyclonal 50 tests
L. pneumophila serogroup 5, polyclonal 50 tests
L. pneumophila serogroup 6, polyclonal 50 tests
L. pneumophila serogroup 7, polyclonal 50 tests
L. pneumophila serogroup 8, polyclonal 50 tests
L. pneumophila serogroup 9, polyclonal 50 tests
L. pneumophila serogroup 10, polyclonal 50 tests
L. pneumophila serogroup 11, polyclonal 50 tests
L. pneumophila serogroup 12, polyclonal 50 tests
L. pneumophila serogroup 13, polyclonal 50 tests
L. pneumophila serogroup 14, polyclonal 50 tests

Legionella pneumophila FITC conjugated antibodies
A full range of Legionella pneumophila FITC conjugated antibodies:

L. pneumophila serogroup 2 0.5 ml
L. pneumophila serogroup 3 0.5 ml
L. pneumophila serogroup 4 0.5 ml
L. pneumophila serogroup 5 0.5 ml
L. pneumophila serogroup 6 0.5 ml
L. pneumophila serogroup 7 0.5 ml
L. pneumophila serogroup 8 0.5 ml
L. pneumophila serogroup 9 0.5 ml
L. pneumophila serogroup 10 0.5 ml
L. pneumophila serogroup 11 0.5 ml
L. pneumophila serogroup 12 0.5 ml
L. pneumophila serogroup 13 0.5 ml
L. pneumophila serogroup 14 0.5 ml

All of the above single reagents are monovalent, polyclonal antibodies

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Listeria

Listeria Unique™
Listeria Visual Immunoassay
Listeria Biochemical ID Test System

Why test your product and production environment for Listeria?

Listeria spp. occur in a range of different environments and can be found as contaminants in a wide variety of foods including meat products, poultry, egg products, cheese, milk, ice cream and other dairy products, frozen and processed seafood, confectionery, vegetables, and fruit.
Listeria monocytogenes and Listeria ivanovii are known human pathogens. These organisms are found in the food manufacturing environment where product crosscontamination, before and following processing, has been reported.
The ubiquitous nature of Listeria spp. also means that they are important indicator organisms for plant hygiene.
Listeria is recognised by food safety regulators as one of the most important contaminants of food. Consequently, some government regulators require environmental and end product testing for Listeria in the food industry.
Listeria has been the cause of a large number of food-poisoning outbreaks leading to major product recalls. Illness, and in some cases death, has resulted from these outbreaks. L. monocytogenes is of particular concern for pregnant women because it can cause abortion or still birth. This food-borne pathogen can also lead to meningitis and brain damage.
The Listeria Visual Immunoassay method makes testing for Listeria fast, simple, accurate, reliable and economical.

Listeria Unique™
Listeria Unique™ is a novel method for fast, convenient and reliable detection of Listeria spp. in food, food related and environmental samples using immunoenrichment™ technology.

Fast

Next Day Result!
Rapid results ensure product can be released sooner, reducing holding costs.

Convenient

One simple enrichment step, saving you time and money.
Only UNIQUE™ offers manual use or ‘walk-away’ automation with the same test module.
Minimal hands-on time.
All reagents provided in ready-to-use self contained modules.
Compact modules for easy storage.
Support tray for handling and storing modules.

Reliable

Validated for a wide range of food and environmental samples.
High sensitivity: 1 cfu / 25 g sample.
Immunoenrichment™ technology significantly reduces background flora, making Listeria isolation extremely reliable.

Built in positive and negative controls give you confidence!
Easy-to-read results

Listeria Visual Immunoassay
A rapid and specific screening test for the detection of Listeria spp. in food and environmental samples.

Fast

You can screen out negative samples within 48 hours. No need to wait up to 6 days, as with conventional methods.
Rapid results ensure inventory can be released earlier, reducing holding costs.

Added Confidence

Listeria VIA™ will detect L . monocytogenes and L. ivanovii, the two nown Listeria human pathogens.
Note: L. monocytogenes specific tests will notdetect L. ivanovii.

Simple and conveniet

All reagents provided.
Easy-to-read results.
The Enviroswab™ ensures optimal recovery and results for environmental samples when used with the kit.

Accurate & Reliable

High affinity antibodies give results in which you can have confidence.
Sensitivity: 1-5 cfu / 25 g sample
Specificity: > 99%
New enrichment protocols for all foods are available using a specially formulated Listeria enrichment broth, designed to improve the recovery of sublethally damaged cells.

Proven Performance

Comparative studies show performance that is equivalent to standard cultural methods.

Economical

The simplicity of the Listeria VIA™ method frees up laboratory time for other tasks and projects. More can be accomplished with the same labor.

Flexible

Recommended for a wide range of food and environmental samples.
Depending on your specific needs, the kit can be used manually or automated for large scale testing.

Listeria Biochemical ID Test System
For the identification of Enterobacteriaceae and Listeria spp.

The Listeria Biochemical ID Test System can be used to confirm the identity of Listeria spp.

Current Database
One of the major challenges facing microbiologists is keeping up to date with changes in taxonomy. All recent changes are incorporated into the Identification System Software.

Economical
ID kits can save time and money, eliminating the need to make up numerous types of biochemical test media.

Flexible
A single software package for both ID kits.

Proven Performance & Reliability
Comparative studies show greater accuracy and reliability than other biochemical ID kits currently on the market

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Pseudomonas

Pseudomonas Visual Immunoassay

Pseudomonas Visual Immunoassay
A rapid and specific screening test for the detection of Pseudomonas spp. in
food, water, cosmetic and pharmaceutical products and environmental
samples.

Pathogen & Spoilage Organisms
Contamination of cosmetics, toiletries and pharmaceuticals by Pseudomonas spp. is well documented. Pseudomonas spp. have been linked to consumer infection and product spoilage, resulting in substantial economic loss.

In the European Union, P.aeruginosa must not be detectable in 0.5 g or 0.5 ml of cosmetic products for the eye area, mucous membranes or for cosmetic products specifically intended for children under 3 years. In other cosmetics, P.aeruginosa must not be detectable in 0.1 g or 0.1 ml (EC 1999).

Pseudomonas spp. are associated with the spoilage of a range of food types including dairy products, eggs, fish, meat and vegetables.

Water has been associated with outbreaks or sporadic cases of foodborne illness resulting from Pseudomonas spp. 2 Studies have shown Pseudomonas spp. to be present in ground and spring water, municipal treated water, filters and softeners and bottled water.

The Australasian Bottled Water Association (ABWI) has set standards (ABWI Model Code) stating that P.aeruginosa shall not be detected in five 250 ml samples.

Fast
Using the Pseudomonas Visual Immunoassay (VIA™), you can:

Detect P. aeruginosa in only 24 hours.
Detect Pseudomonas spp. in only 48 hours! No need to wait up to 7 days, as with standard cultural methods.
Ship product sooner based on a rapid result.

Reliable

High affinity antibodies give results in which you can have confidence.
High sensitivity: 1-5 cfu / 1 g of sample.
Recommended for a wide range of raw materials, finished products, water and environmental samples and foods.
Comparative studies showed performance equivalence to standard cultural methods.

Convenient

All reagents provided ready-to-use.
Easy-to-read results.
A single enrichment broth may be used to screen for both.
Pseudomonas spp. and Staphylococcus aureus, using the Pseudomonas VIA and the Staphylococcus aureus VIA.
The simplicity of the Pseudomonas VIA method frees up laboratory time for other tasks and projects.
Depending on your specific needs, the kit can be used manually or automated for large scale testing

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Salmonella

Salmonella Unique™
Salmonella Ultima™
Salmonella Visual Immunoassay

Why test for Salmonella?

Salmonella is regarded as one of the most serious threats to producers and consumers of food, all over the world.
Food poisoning outbreaks involving this pathogen have resulted in major product recalls and substantial economic loss. Numerous cases of illness and many deaths have resulted from these outbreaks.
Food poisoning outbreaks caused by Salmonella spp. have involved a wide range of foods including, meat and meat products, poultry, eggs, milk and dairy products, fish, shrimp, frog legs, yeast, coconut, sauces and salad dressing, cake mixes, cream-filled desserts and toppings, dried gelatin, peanut butter, cocoa, chocolate and pasta.
It is estimated that up to 4 million cases of salmonellosis occur in the U.S. annually. The incidence of salmonellosis appears to be rising in the U.S. and other industrialised countries.
The Visual Immunoassay (VIA) method makes testing for Salmonella fast, simple, reliable and economical.
Salmonella Unique™
Fast, Convenient, Reliable Salmonella Detection with Immunoenrichment™ technology

Fast

From sample to result in less than 22 hours!
Rapid results ensure product can be released sooner, reducing holding costs.

Convenient

Reliable

Extensively tested for a wide range of food and environmental samples
High sensitivity: 1 cfu / 25 g sample.
Immunoenrichment™ technology significantly reduces background flora, making Salmonella isolation extremely reliable.
Detects motile and non-motile Salmonella.
No toxic selective enrichment media required.

Built in positive and negative controls give you confidence
Easy-to-read results

Unique™ Salmonella AOAC Official Method 2000.07 Collaborative Study Summary

Trial Parameters: 41 collaborators
3 inoculum levels:
Uninoculated
Low Level (1-5 cells / 25 g)
High level (10-50 cells / 25 g)
1200 food samples tested.
Outcome: The UNIQUE™ Salmonella method is equivalent to the US FDA BAM Reference Method.

Unique™ Salmonella gave 100% inclusivity and 100% exclusivity in validation trials2
Summary: 91 strains of Salmonella representing 81 serotypes were tested and shown to be positive. 29 strains of non-Salmonella from 23 species were tested and gave negative results.

Salmonella Ultima™
Rapid, Convenient Salmonella Detection with an advanced Visual Immunoassay for screening food, food related and environmental samples.

The simplicity of Salmonella ULTIMA
All foods

25 g sample + 225 ml Buffered Peptone Water.
35-37 ºC, 16-20 hours
0.1 ml.
10 ml Rappaport-Vassiliadis R10 Broth or Rappaport-Vassiliadis Soy Peptone Broth.
41-43 ºC, 18-24 hours.
Salmonella Ultima™

Rapid

Results in less than 36 hours!

Cost Effective

Less labour time per test compared with Standard Methods and other rapid methods that use M-broth and 2 selective enrichments.
Save money on culture media and laboratory consumables.
Convenient
One simple enrichment protocol for all food.
Simple two-step enrichment.
No M-broth or secondary enrichment required.
Single selective enrichment broth.
Results easily read by eye with option of semi-automated or fully automated testing.

Reliable

Evaluated for a wide range of food and environmental samples.
High Sensitivity : 1-5 cfu / 25 g sample.
Detects motile and non-motile Salmonella.

Trial Results Show Salmonella ULTIMA to be more sensitive than ISO Reference method.
Reference Method: ISO # 6579

Trial Parameters: 20 food types
140 samples
20 Salmonella strains
2 inoculum levels: Uninoculated
Low Level (1-5 cells / 25 g sample)

Outcome: Salmonella ULTIMA showed a zero false positive rate and a zero false negative rate. The ISO Reference method gave a zero false positive rate and 4 false negative results.

The results generated in this study support the applicability of Salmonella ULTIMA to a wide range of food types including raw and processed meats (including chicken), dairy products, egg products, confectionery, seafood, fruit and yeast products.

Salmonella Visual Immunoassay
A rapid and specific screening test for the detection of Salmonella spp. in food and environmental samples.

Fast

Results available within 42 hours compared to 4 days for standard cultural methods.
You can ship product sooner based on a rapid result.
Simple and convenient
No membrane strip which may become clogged with sample residue and affect the test result.
All reagents provided.
Easy-to-read results.
The option of using a single selective enrichment step, saving time and money.
Accurate & Reliable
Unique high affinity antibodies give results in which you can have confidence.
Sensitivity : 1-5 cfu / 25 g sample
Specificity : 95-100%
Independent studies have shown the method to be more sensitive than comparative rapid methods.

Proven Performance

Comparative studies show performance that is equivalent to standard cultural methods.

Economical

The simplicity of the Salmonella VIA method frees up laboratory time for other tasks and projects. More can be accomplished with the same labor.
Rapid results ensure you can release inventory earlier, reducing holding costs.

Flexible

Recommended for a wide range of food, food-related and environmental samples.
Depending on your specific needs, the kit can be used manually, semi-automated or fully automated for large scale testing.

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Staphylococcus

Prolex Staph Latex Kit
Coagulase plasma, 2 ml, 5 ml, 10 ml or 20 ml
Staphylococcus Aureus Visual Immoassay

Prolex™ Staph Latex Kit
The Prolex™ Straphylococcus aureus Kit provides a sensitive rapid latex agglutination assay for the identification of S. aureus by detecting coagulase (clumping factor) and/or Protein A.

ProlexTM Staphylococcus aureus Kit Features

Rapid results obtained within seconds!
Detection of coagulase and Protein A ensures optimum specificity for a wide range of Staphylococcus aureus strains
100% sensitivity!
All components are colour-coded for ease in identification
Colour-coded reagent bottles
Ingenious stackable workstation packaging allows easy reagent access and minimal utilization of refrigerator space
Comes complete with negative control, disposable cards, and mixing sticks
Available in two convenient 100 and 300 test sizes

Coagulase plasma, 2 ml, 5 ml, 10 ml or 20 ml

Lyophilized. Rabbit plasma w/EDTA. For detection of coagulase enzyme in staphylococci.

Coagulase Plasma

10 X 2 ml vial
10 X 5 ml vial
10 ml vial
20 ml vial
30 ml vial

Coagulase test
The enzyme coagulase, produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection off from the host's protective mechanisms rather effectively.

There are 2 methods: slide test and tube test. The tube test is more accurate, but the slide test is faster. If you have a Staph unknown, you might consider running the slide test, and, if negative, running the tube test. The tube method is THE definitive test.

Objectives
Identify the presence of the enzyme coagulase.

Materials needed
0.5 ml rabbit plasma per unknown

The procedure
Pathogenic Staph species (e.g. Staph aureus) can be confirmed using the coagulase test. Since there are 2 kinds of coagulase enzyme—bound and free---there are 2 different tests that can be used to identify these enzymes. Both of the enzymes activate fibrinogen in plasma, in different ways.

Slide Agglutination test
Make a 2,54 cm diameter circle on a clean glass slide using a wax pencil. Place two drops of thawed rabbit plasma into the circle, using a wooden pick or a clean loop. Add a single colony and emulsify it in the plasma. Add a single colony and emulsify it in the plasma. Fibrin threads form between the cells, causing them to agglutinate, or clump. There will a visible clumping of cells within 10-15 seconds. This test is for the bound coagulase enzyme.
Tube Agglutination test
Inoculate a tube with a 0,5 ml of rabbit plasma with the bacterial inoculum. Place at 37 °C and check at 0,5 hour and after (some strains will give a + reaction in a few hours, other strains take longer) by tipping the slide at an angle. Any degree of coagulation is considered a positive test for the free coagulase enzyme. Add a single colony and emulsify it in the plasma. Fibrin threads form between the cells, causing them to agglutinate, or clump. There will a visible clumping of cells within 10-15 seconds. This test is for the bound coagulase enzyme.

Staphylococcus Aureus Visual Immoassay
A rapid and specific screening test for the detection of Staphylococcus aureus in food, cosmetic and pharmaceutical products, and environmental samples.

Staphylococcal food poisoning is one of the most common types of foodborne disease worldwide. Food poisoning outbreaks caused by S. aureus have involved a wide range of foods including meat, poultry and egg products, cream-filled bakery products and dairy products.

Because of the pathogenicity of S. aureus, the United States Pharmacopeia (USP) requires its absence in certain nonsterile pharmaceutical raw materials and finished products (U.S. Pharmacopeia 1995).

In the European Union, S. aureus must not be detectable in 0.5 g or ml of cosmetic products specifically intended for: children under 3 years, eye area and mucous membranes. In other cosmetics, S. aureus must not be detectable in 0.1 g or ml (EC 1999).

Fast

You can screen out negative samples within 26 hours. No need to wait up to 6 days, as with standard cultural methods.
You can ship product sooner based on a rapid result

Reliable

Highly purified antibodies give results in which you can have confidence.
Sensitivity:

1-5 cfu / 3 g of food
1-5 cfu / 1 g of cosmetics and pharmaceuticals
By performing the appropriate dilution prior to enrichment, you can obtain a semi-quantitative result to suit your needs.
Recommended for a wide range of food, cosmetic and pharmaceutical samples.

Proven Performance

Comparative studies show performance that is at least equivalent to standard cultural methods.

Convenient

All reagents provided.
Easy-to-read results.
A single enrichment broth can be used to screen for S. aureus and Pseudomonas spp., using the Staphylococcus aureus VIA and the Pseudomonas VIA.
The simplicity of the Staphylococcus aureus VIA method frees up laboratory time for other tasks and projects.
Depending on your specific needs, the kit can be used manually or automated for large scale testing.
Each sample goes into a single well, making this method more cost effective than cultural methods, which require 10 plates per 1 g sample (at a 1/10 dilution). This means the Staphylococcus aureus VIA is a more efficient method, particularly for testing:

large sample sizes; and/or
multiple sample

Enterotoxin Testing & Identification
Some strains of S. aureus produce enterotoxins, which show a high degree of thermal stability when subjected to heat treatments commonly used in food processing to kill viable cells. Therefore, it is advisable that foods be examined for the presence of residual toxin using a Staphylococcal Enterotoxin detection kit.

The same enrichment broth, used to detect S.aureus, can also be tested for Staphylococcal Enterotoxins using the Staphylococcal Enterotoxin VIA or UNIQUE™ Staphylococcal Enterotoxin assay.
Individual toxins can then be identified using the Staphylococcal Enterotoxin Identification VI

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Streptococcus

Prolex Strep New & Blue Latex Kit

Prolex Strep New & Blue Latex Kit
A unique streptococcal grouping latex agglutination kit for the rapid identification of beta-hemolytic streptococci of groups A, B, C, F, G and D from primary culture plates. Prolex’s use of a modified nitrous acid extraction has allowed the kit to offer a group D latex to be used in the same convenient manner as the other group latex.

Rapid

Immediate modified nitrous acid extraction procedure
Accurate results in one minute at room temperature

Sensitive

As little as one colony required

Convenient

All colour-coded reagents are ready-to-use and remain in only one space-saving workstation.

Easy-to-read

Blue latex particles on a white reaction card, create one easy-to-read result.

Flexible

Strep-Select option allows you to select the latex reagents you want for your requirements.

Prolex™ Streptococcal Grouping Kit Features

Identification of groups A, B, C, F, G, and D!
Easy-to-interpret positive and negative results
Complete procedure performed at room temperature within 2 minutes
All reagents are liquid ready-to-use
Available in convenient kit format or separate components for ultimate convenience and economy
All components are colour-coded for ease in identification
No special equipment required
Colour-coded extraction reagents provide a visual check on the extraction procedure
Ingenious stackable workstation packaging allows for ease in reagent access and minimal utilization of refrigerator space

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Unique Plus™ Instrument
UNIQUE PLUS™ is an instrument designed for affordable automated pathogen screening. Only one simple enrichment step, then place the UNIQUE™ module into the UNIQUE PLUS™ and walk away!

True ‘walk away’ automation
Suitable for small to medium scale testing
Run 1 to 30 samples at a time
Independently validated and approved
Simple to use
Automated Immunoenrichment™ eliminates multiple selective enrichment steps
Results on a printout or downloaded on to a computer
Built-in incubator
Robust design with minimal moving parts
Reduced labour costs; frees up staff for other tasks
2 year warranty on parts and labour
Different assays are available to run on the UNIQUE PLUS™
Now available:

SALUNQ
LISUNQ
SETUNQ
CAMUNQ

Soon to be available

ECOUNQ

Automated pathogen screening in 5 easy steps:
1. Insert the appropriate Protocard™ into the UNIQUE PLUS™ and enter sample information.
2. Place the modules into the UNIQUE PLUS™ tray.
3. Transfer 1 ml of enrichment broth into tube 1.
4. Insert a UNIQUE stick into each module and place the tray into the UNIQUE PLUS™ instrument.
5. Press 'Start' and walk away. Collect results at your convenience, within hours!

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Product List

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