Identification & Confirmation Tests
- Captivate™ E.coli
- Captivate™ Salmonella
- Prolex™ E. coli O157 Latex kit, E. coli 0157:H7 Latex Test Reagent 2 ml
- Prolex™ Legionella Latex Kit
- Prolex™ Legionella Individual Latex Reagents
- Legionella pneumophila FITC conjugated antibodies
- Prolex Staph Latex Kit
- DiaMondiaL Staph Plus, 4th generation
- Coagulase plasma, 2 ml, 5 ml, 10 ml, 20 ml or 30 ml
Captivate™
Captive™ is a range of antibody coated paramagnetic particles for the specific ImmunoMagneticSeparation of microorganisms.
This technology consists of microscopic paramagnetic particles. The beads have a magnetite core and a “ceramic” zirconium oxide coating. The beads are manufactured by high speed blending process and typically cover a size diameter range of 1-4 µm, with a 2.5 µm average size.
Purified antibodies to surface components of the target microorganism are covalently coupled to the bead. With careful antibody selection, a highly specific separation system for microorganisms is produced.
The pre-coated beads are designed for the IMS of target bacteria from enrichment cultures. A sample is taken form a filter stomacher bag and incubated with the Captive™ beads for 30 minutes. The bead/microoragnism complexes are then removed from the sample by placing the sample in a magnetic concentrator device. This separates them form the background organisms and interfering materials. The complexes are then washed using a PBS/Teen 20 wash buffer to remove non-specifically bound material. The beads can then be plated out onto the appropriate selective agar media and incubated as described.
The IMS technique will increase the sensitivity of the methodology and, in most circumstances, results can be achieved 24 hours earlier than standard protocols.
These products can also serve as a capture system for rapid detection systems.
Special Notes on IMS Techniques.
There are important factores that affect the performance of IMS techniques. Thorough mixing of the particles and sample allied with efficient recovery of the beads from the sample matrix is paramount to the success of this technique. Care must be taken not to aspirate the sample vigorously as this can result in the loss of captured target organisms. Certain sample types (e.g. very fatty, particulate and viscous samples) can interfere with bead recovery. To counter-act this interference, samples can also be diluted in PBS-Tween e.g. 1:2-1:4, reducing the effect of the matrix and allowing more efficient bead recovery. Alternatively with problem samples, after the initial magnetic separation the incomplete removal of the sample (i.e. remove 800 µl) and continuation of the wash protocol as described can minimise bead losses.
Captivate™ E.coli
Captive™O157
Captive™O157 are magnetisable particles coated with specific antibody intended for the isolation of E.coli O157:H7 from food, animal feeds, beverages, pharmaceutical or environmental samples. The particles help to concentrate O157:H7 cells in mixed culture reducing the probability of missing low numbers or overgrowth of O157:H7 colonies by competing flora. In fact, immunomagnetic separation is now regarded as the gold standard method for isolation of E.coli O157:H7 from food and environmental samples.
E.coli O157:H7 is the primary serovar associated with food borne gastrointestinal infection, resulting in self-limiting diarrhoea, that can lead to serious disease conditions such as haemorrhagic colitis, haemolytic uraemic syndrome and thrombotic thrombcocytopaenic purpura (TTP).
The organism itself is associated with raw meats and unpasteurised milk, probably due to the implication of farm animals and particularly cattle as carriers of E.coli O157:H7. Large outbreaks have been recorded in the United States from consumption of unpasteurised apple juice (apple cider) possibly as a result of using apples which have fallen to the ground where the potential for contamination with the organism exists.
Also available
Captivate™O26
Captivate™O111
Captivate™O103
Captivate™O145
Captivate™ Salmonella
This product is designed to capture and concentrate the common serotypes of Salmonella involved in human and animal disease from enriched samples. The particles are coated with affinity purified plyclonal antibody directed towards, common somatic and flagellar antigens. This gives an excellent high avidity broad spectrum IMS reagent fro the capture of salmonellas. Due to the large variation in Salmonella serotypes and antigen expression there is naturally strain dependent variation in the capture efficiency.
E. coli
Why test for E. coli O157?
E. coli O157 is regarded as one of the most serious threats to the producers and consumers of food, all over the world. Food poisoning outbreaks, caused by E. coli O157, have involved a wide range of foods including meat and meat products, milk and dairy products, vegetables, salads and fruit juices.
Outbreaks involving E. coli O157 have resulted in major product recalls and substantial economic loss. Numerous cases of illness and many deaths have resulted.
The USDA has issued an E. coli O157:H7 test directive to producers, retailers and
importers/exporters of raw ground beef products.
E. coli O157 Latex kit, E. coli O157:H7 Latex Test Reagent 2 ml
The innovative E.coli O157 latex kit offers definitive identification of E.coli O157 through the use of a proprietary antibody that eliminates the possibility of cross-reactions with Escherichia hermanii and other sorbitol-negative Escherichia species.
So sensitive, only a single, direct colony is required
Rapid results in only 2 minutes!
Presented in a uniquely designed, user friendly workstation
Available in two convenient sizes of 50 or 100 tests
Contains positive and negative control
Incredible 2 year shelf life!
Prolex™ E. coli H7 Flagellar Antigen Latex Reagent
The use of the Prolex™ H7 reagent allows immediate identification and confirmation of positive E. coli O157:H7 organisims.
Legionella
Prolex Legionella Latex Kit
bioTRADING offers a kit employing a monoclonal antibody, specifically directed against surface antigens on Legionella pneumophila serogroup 1, eliminating cross reactions with other pneumophila and non pneumophila serogroups as well as other species of bacteria. The kit is supplied complete with test latex, positive and negative controls, reaction cards and mixing sticks, sufficient for 20 tests.
Prolex Legionella Individual Latex Reagents
A full range of Legionella Individual Latex Reagents:
L. pneumophila serogroup 2, polyclonal 50 tests
L. pneumophila serogroup 3, polyclonal 50 tests
L. pneumophila serogroup 4, polyclonal 50 tests
L. pneumophila serogroup 5, polyclonal 50 tests
L. pneumophila serogroup 6, polyclonal 50 tests
L. pneumophila serogroup 7, polyclonal 50 tests
L. pneumophila serogroup 8, polyclonal 50 tests
L. pneumophila serogroup 9, polyclonal 50 tests
L. pneumophila serogroup 10, polyclonal 50 tests
L. pneumophila serogroup 11, polyclonal 50 tests
L. pneumophila serogroup 12, polyclonal 50 tests
L. pneumophila serogroup 13, polyclonal 50 tests
L. pneumophila serogroup 14, polyclonal 50 tests
Legionella pneumophila FITC conjugated antibodies
A full range of Legionella pneumophila FITC conjugated antibodies:
L. pneumophila serogroup 2 0.5 ml
L. pneumophila serogroup 3 0.5 ml
L. pneumophila serogroup 4 0.5 ml
L. pneumophila serogroup 5 0.5 ml
L. pneumophila serogroup 6 0.5 ml
L. pneumophila serogroup 7 0.5 ml
L. pneumophila serogroup 8 0.5 ml
L. pneumophila serogroup 9 0.5 ml
L. pneumophila serogroup 10 0.5 ml
L. pneumophila serogroup 11 0.5 ml
L. pneumophila serogroup 12 0.5 ml
L. pneumophila serogroup 13 0.5 ml
L. pneumophila serogroup 14 0.5 ml
All of the above single reagents are monovalent, polyclonal antibodies
Listeria
Why test your product and production environment for Listeria?
Listeria spp. occur in a range of different environments and can be found as contaminants in a wide variety of foods including meat products, poultry, egg products, cheese, milk, ice cream and other dairy products, frozen and processed seafood, confectionery, vegetables, and fruit.
Listeria monocytogenes and Listeria ivanovii are known human pathogens. These organisms are found in the food manufacturing environment where product crosscontamination, before and following processing, has been reported.
The ubiquitous nature of Listeria spp. also means that they are important indicator organisms for plant hygiene.
Listeria is recognised by food safety regulators as one of the most important contaminants of food. Consequently, some government regulators require environmental and end product testing for Listeria in the food industry.
Listeria has been the cause of a large number of food-poisoning outbreaks leading to major product recalls. Illness, and in some cases death, has resulted from these outbreaks. L. monocytogenes is of particular concern for pregnant women because it can cause abortion or still birth. This food-borne pathogen can also lead to meningitis and brain damage.
Salmonella
Why test for Salmonella?
Salmonella is regarded as one of the most serious threats to producers and consumers of food, all over the world.
Food poisoning outbreaks involving this pathogen have resulted in major product recalls and substantial economic loss. Numerous cases of illness and many deaths have resulted from these outbreaks.
Food poisoning outbreaks caused by Salmonella spp. have involved a wide range of foods including, meat and meat products, poultry, eggs, milk and dairy products, fish, shrimp, frog legs, yeast, coconut, sauces and salad dressing, cake mixes, cream-filled desserts and toppings, dried gelatin, peanut butter, cocoa, chocolate and pasta.
It is estimated that up to 4 million cases of salmonellosis occur in the U.S. annually. The incidence of salmonellosis appears to be rising in the U.S. and other industrialised countries.
Staphylococcus
Prolex™ Staph Latex Kit
The Prolex™ Straphylococcus aureus Kit provides a sensitive rapid latex agglutination assay for the identification of S. aureus by detecting coagulase (clumping factor) and/or Protein A.
ProlexTM Staphylococcus aureus Kit Features
Rapid results obtained within seconds!
Detection of coagulase and Protein A ensures optimum specificity for a wide range of Staphylococcus aureus strains
100% sensitivity!
All components are colour-coded for ease in identification
Colour-coded reagent bottles
Ingenious stackable workstation packaging allows easy reagent access and minimal utilization of refrigerator space
Comes complete with negative control, disposable cards, and mixing sticks
Available in two convenient 100 and 300 test sizes
Coagulase plasma, 2 ml, 5 ml, 10 ml or 20 ml
Lyophilized. Rabbit plasma w/EDTA. For detection of coagulase enzyme in staphylococci.
DiaMondiaL Staph Plus, 4th generation, DML1100 (100 tests) or DML1130 (300 tests)
Dutch study, March 2009: 'The DML Staph PLUS Latex Kit has a perfect positive predictive value in this collection of S.aureus strains, both sensitive (MSSA) and resistant (MRSA) strains.
Product Features
Enhanced results with blue carboxylated microparticles (CM-MP)
Detects Coagulase, Protein A
Detects Capsular Polysaccharide 5 and 8
Definitive results obtained within 20 seconds
High performance with sensitivity of 100% and specificity of 100%
Contains negative control
Blue Latex
White reaction cards
DiaMondiaL Staph Plus Latex Kit utilizes Carboxylate Modified Microparticles!
Coagulase Plasma
- 10 X 2 ml vial
- 10 X 5 ml vial
- 10 ml vial
- 20 ml vial
- 30 ml vial
Coagulase test
The enzyme coagulase, produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection off from the host's protective mechanisms rather effectively.
There are 2 methods: slide test and tube test. The tube test is more accurate, but the slide test is faster. If you have a Staph unknown, you might consider running the slide test, and, if negative, running the tube test. The tube method is THE definitive test.
Objectives
Identify the presence of the enzyme coagulase.
Materials needed
0.5 ml rabbit plasma per unknown
The procedure
Pathogenic Staph species (e.g. Staph aureus) can be confirmed using the coagulase test. Since there are 2 kinds of coagulase enzyme—bound and free---there are 2 different tests that can be used to identify these enzymes. Both of the enzymes activate fibrinogen in plasma, in different ways.
Slide Agglutination test
Make a 2,54 cm diameter circle on a clean glass slide using a wax pencil. Place two drops of thawed rabbit plasma into the circle, using a wooden pick or a clean loop. Add a single colony and emulsify it in the plasma. Add a single colony and emulsify it in the plasma. Fibrin threads form between the cells, causing them to agglutinate, or clump. There will a visible clumping of cells within 10-15 seconds. This test is for the bound coagulase enzyme.
Tube Agglutination test
Inoculate a tube with a 0,5 ml of rabbit plasma with the bacterial inoculum. Place at 37 °C and check at 0,5 hour and after (some strains will give a + reaction in a few hours, other strains take longer) by tipping the slide at an angle. Any degree of coagulation is considered a positive test for the free coagulase enzyme. Add a single colony and emulsify it in the plasma. Fibrin threads form between the cells, causing them to agglutinate, or clump. There will a visible clumping of cells within 10-15 seconds. This test is for the bound coagulase enzyme.
Streptococcus
DiaMondiaL Strep Kit
A unique streptococcal grouping latex agglutination kit for the rapid identification of beta-hemolytic streptococci of groups A, B, C, F, G and D from primary culture plates. DiaMondiaL's use of a modified nitrous acid extraction has allowed the kit to offer a group D latex to be used in the same convenient manner as the other group latex.
Rapid
Immediate modified nitrous acid extraction procedure
Accurate results in one minute at room temperature
Sensitive
As little as one colony required
Convenient
All colour-coded reagents are ready-to-use and remain in only one space-saving workstation.
Easy-to-read
Blue latex particles on a white reaction card, create one easy-to-read result.
Flexible
Strep-Select option allows you to select the latex reagents you want for your requirements.
DiaMondiaL Strep Grouping Kit Features
Identification of groups A, B, C, F, G, and D!
Easy-to-interpret positive and negative results
Complete procedure performed at room temperature within 2 minutes
All reagents are liquid ready-to-use
Available in convenient kit format or separate components for ultimate convenience and economy
All components are colour-coded for ease in identification
No special equipment required
Colour-coded extraction reagents provide a visual check on the extraction procedure
Please contact us if you need a specific product, we are meeting many customers’ unique requests.
