Culture Media
Dehydrated Culture Media
bioTRADING supplies high-quality dehydrated culture media for clinical, industrial, biotech and life science applications. We are able to offer high-value dehydrated culture media of superior and consistent quality, as well as unparalleled service to our customers.
We are able to address all aspects of providing our customers with product of high value, no matter how it is defined — be it quality, delivery, availability, service or cost.
Quality Assurance
Detailed certificates of origin are available for every product and lot number. It is the policy of bioTRADING to achieve the highest degree of product quality.
Service
bioTRADING is fully committed to maintaining the inventory necessary to fill a customer’s needs — on the customer’s timetable. Having our varied media products in stock, and continuously available for same day and next-day shipments, is what our customers have come to expect from us, and what we expect from ourselves. Should questions arise about the correct usage of any of our products, we stand behind them with professional technical support.
Value
bioTRADING provides the greatest value dehydrated culture media by supplying:
- Consistency from lot-to-lot
- Availability
- On-time delivery
- Outstanding customer and technical service
One of the basic characteristics of a micro organism is its nutritional requirements. A dehydrated culture medium is described as a substance or a group of substances that satisfies those nutritional requirements.
With many simple and complex formulations of dehydrated culture media available, it is important for a media supplier to supply the finest product possible.
Supplements, selective- & growth-
Lyophilised supplements are presented in packs of 10 vials, and for the majority of the supplements each vial is sufficient for 500 ml of medium. Larger and smaller volumes are indicated for relevant products.
The shelf life of freeze-dried supplements is 2-3 years provided they are stored in a refrigerator at 2-8 °C. Once rehydrated the stability of antibiotics varies greatly and will determine the shelf life of the prepared agars and broths. For this reason any unused, rehydrated, supplement should be discarded, as even deep-freezing may not prevent the rapid degradation of the antibiotics. To ensure the correct level of selective supplements the entire vial contents must be added to the stated volume of cooled, molten medium.
Rehydration
Vials should be rehydrated aseptically using a sterile needle and syringe charged with 5 ml of the specified diluent for the particular supplement being added. The supplement should be rehydrated, withdrawn and added to the medium in a single process, followed by immediate disposal of the syringe into an approved container. Under no circumstances attempt to re-sheath an exposed neeldle.
Addition
Most antibiotics are heat labile, and so to prevent a reduction of potency the medium should be cooled to 47 °C by holding in a water bath set at this temperature.
Once the supplement has been added the medium must be gently but thoroughly mixed to ensure that the selective agents are evenly distributed. Failure to do this will result in a range of concentrations in the plates/bottles and consequent inconsistency in results. The shelf life of supplemented media is governed by the stability of the added components, and is generally shorter than unsupplemented agars and broths. For information on the shelf life of prepared media consult the individual product listings in the previous section of the manual.
Ready Prepared Culture Media
Based in Mijdrecht The Netherlands, bioTRADING is a leader in the manufacture of classic and custom media. bioTRADING provides products manufactured to international standards or your specifications using your formulation, fill volume and configuration. bioTRADING is capable of accommodating your unusual plate, tube, bottle or bag configurations. bioTRADING quality control testing can be designed to use your specific requirements too. Organism challenges and sterility testing can be tailored to duplicate your present in-house methods.
bioTRADING is ISO 9001:2008 certified
Equipment
In our purpose built facility we have a wide variety of modern equipment, validated in accordance with international standards/guidelines. Our autoclaves are not just ordinary steam autoclaves, but they are specially designed; giving us reduced heating up and cooling down time; facilitating in the correct treatment of media, "not over-cooking media" as is sometimes the case in certain circumstances.
Quality Control
Every batch is rigorously tested to meet performance criteria prior to reaching the laboratory bench top!
Quality Control sheets will provide documented testing results and can be downloaded using a login password.
Pharmaceutical product range.
We supply all media according to the Harmonized USP/EP/JP to perform Sterility and Microbiological Tests.
Ready Prepared Media is our Culture!
We can supply you with what you want, "first-time" -custom-made media if necessary- Quality and Service. If there is a formula that can be made by anyone, why not leave it to us as we can do it too!
We have a "can do" approach and we offer you every deviation available to standard criteria products, such as:
- Test media like FTM, TSB, Rinsing liquids according to Harmonized USP/ EP/JP or your own formulation.
- Media according to ISO 11133 standard.
- Contact plates (30 or 55 mm diameter) such as TSA+tween+lecithin, SDA, MacConkey or one of the many other formulations currently available.
- 55, 90, 100, 120, 150 mm plates.
- 2, 3 or 4-compartment (round or square) plates.
- Plates packed in multiples of 2, 5, 7 or 10 pcs.
- Clean room (multiple) packed in plastic boxes and/or bags.
- Irradiated/non-irradiated.
- Ethylene Oxide-treated.
- Media to be tested with a special strain.
- Media to be tested using an alternative method.
- We offer 30 different deviations of a Blood agar plate.
- Media in tubes with snap cap or screw cap.
- Media in bottles with flip tear-off cap or screw cap including septum.
- Media in bags of 5 liter, like TSB, BPW, Saline, etc.
- Media in jars with screw cap
ColorexTM
Ready to use plates made with the original CHROMagar powder base
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Clinical Microbiology
Colorex Orientation |
Food and Water Industry
Colorex O157 |
Please contact us if you need a specific product, we are meeting many customers’ unique requests.
